Inducible Cre recombination is a site-specific recombinase technology that introduces deletions, insertions, translocations, and inversions at specific sites and specific times. Equipped with a well-constructed technology platform and abundant expertise, Creative Biolabs offers you optimized psychiatric disorder modeling services by inducible Cre technologies where you are located.
What is Inducible Cre?
Cre (Cyclization recombination enzyme), a 38KDa protein composed of 343 amino acids, is a member of the integrase family of a site-specific recombinase that was isolated from P1 bacteriophage in 1981. Cre is a site-specific DNA recombinase with not only catalytic activity but also restriction endonuclease-like activity, which can specifically recognize loxP sites and mediate the site-specific deletion or recombination between loxP sites. LoxP (locus of X-overP1) site is a 34bp sequence from P1 bacteriophage, consisting of two 13bp reverse repeats and an 8bp interval sequence, among which the reverse repeats are the specific recognition sites of Cre recombinase, and the interval region determines the direction of the loxP site.
In 1987, the Cre-loxP recombination system was first reported to manipulate specific recombination at specific sites in mitotic and non-mitotic cells, which was initially used to activate gene expression in mammalian cell lines. Subsequently, it has been demonstrated that Cre-loxP recombination could efficiently delete DNA sequences on both sides of loxP in the transgenic animal modeling. To gain more accurate genetic functional studies, animal modeling, and clinical application, the inducible Cre-loxP system was developed. The inducible Cre system is controlled by cell-specific regulatory elements or exogenous inducers in a time-dependent manner to achieve the target gene engineering at a specific time.
Inducible Cre for Psychiatric Disorder Modeling
As one of the most common neurological diseases, psychiatric disorders refer to a wide range of mental health conditions, mainly dysfunctions of cognition, emotion, thinking, and behavior. Inducible Cre recombination is a recently developed technique that enables targeting gene modification to specific brain structures at different times, serving as a useful tool for psychiatric disorder transgenic animal modeling.
For psychiatric disorder modeling, inducible Cre technologies can introduce a gene of interest or target mutations to specific cell types to the brain. Thus, Cre modified mutation is expressed only in certain cells of one mouse. By mating to a mouse that carries a target gene flanked by two loxP sites, the mutation is passed on to the offspring.
Psychiatric Disorder Modeling Services at Creative Biolabs
The Cre-lox system, especially inducible Cre strategies, is a potent and specific system for gene modification. Based on Cre-loxP recombination principles and advanced technology platform, experienced scientists in Creative Biolabs has successfully applied inducible Cre recombination to psychiatric disorder animal modeling. Now we are glad to provide you custom psychiatric disorder modeling services using suitable inducible Cre strategies. And a range of promoter, fusion, or expression system is also available for your choice.
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