Based on the concept of high-quality and time-saving services providing, Creative Biolabs provides psychiatric disorder modeling services by homologous recombination in embryonic stem cells.
Homologous Recombination in Embryonic Stem Cells
Homologous recombination is a type of genetic recombination in which the nucleotide sequences are exchanged between two similar or identical DNA regions on two separate chromosomes or the same chromosome. It usually occurs in eukaryotic cells to accurately repair harmful breaks or eliminate deleterious lesions from chromosomes. The homologous recombination process also produces new combinations of DNA sequences and genetic variation when it occurs in meiosis or zygote formation. Moreover, homologous recombination has recently made a breakthrough in animal gene engineering, accurate human disease modeling, and gene therapy.
The application of homologous recombination in embryonic stem cells, a kind of pluripotent stem cells with great differentiation potential, makes it convenient and efficient to obtain genetic engineering animals. Gene targeting with homologous recombination in embryonic stem cells allows us to manipulate genes with high precision, even in the central nervous system, revolutionizing the research and analysis of neurological disease molecular mechanisms, animal modeling, and drug development.
Homologous Recombination in Embryonic Stem Cells for Psychiatric Disorder Modeling
Psychiatric disorders are an array of neurological conditions characterized by abnormalities in thinking, cognition, emotion, or behavior. Currently, homologous recombination in embryonic stem cells, as an alternative to conventional methods, is increasingly applied for animal modeling of psychiatric disorders.
Firstly, embryonic stem cells are isolated and cultured, then introduce mutations by targeting a new genetic sequence with homologous recombination in embryonic stem cells. Subsequently, the genetically modified embryonic stem cells are injected into a young embryo, which will give birth to chimeras due to their unique ability to colonize all the tissues of a host embryo including its germline. In this way, the embryonic stem cell genotype cells could be 'recycled and expanded' in vivo and transmitted to mutant generations. Psychiatric disorder modeling by homologous recombination in embryonic stem cells can be utilized to create an animal bearing a genetic modification in any cloned gene, not only the introduction of knockout or knockin but also point mutations.
Psychiatric Disorder Services at Creative Biolabs
Aided by our well-established specialized animal modeling platform and experienced scientists, Creative Biolabs is proud of providing global clients with custom animal modeling services for psychiatric disorders. In addition to modeling by homologous recombination, other genetic technologies are also within our abilities, such as Inducible Cre, CRISPR-Cas, and rabies virus-based vectors.
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