Central Nervous System (CNS) and Blood-Brain Barrier (BBB)
Central Nervous System (CNS) is the part of the nervous system consisting primarily of the brain and spinal cord. The CNS is so named because it integrates the received information and coordinates and influences the activity of all parts of the bodies of the bilaterally symmetric animal. Nearly all multicellular animals contain the majority of the nervous system. Blood-Brain Barrier (BBB) is a complex physiological structure formed by the blood vessels of the CNS that tightly regulates the movement of substances between the blood and the neural tissue. Creative Biolabs provides molecular biology methods to clone and express mice with specific gene deletions, which helps you to analyze the transport of substances in the CNS.
The Function of Pgp in Efflux Transport
CNS endothelial cells (ECs) express efflux transporters P-glycoprotein (Pgp/Mdr1/Abcb1) to eliminate potential toxins from the CNS. The mouse genome contains two Pgp genes: Abcb1a and Abcb1b. Thirty-two structurally diverse drugs used for the treatment of various conditions of the CNS, along with two active metabolites, and eight non-CNS drugs were measured in the brain, plasma, and cerebrospinal fluid in the P-glycoprotein (P-gp) knockout mouse model after subcutaneous administration, and the data were compared with corresponding data obtained in wild-type mice. Of the 34 CNS-active agents, only 7 demonstrated brain-to-plasma (B/P) area under the plasma concentration curve ratios between P-gp knockout and wild-type mice that did not differ significantly from unity.
The Function of Slc7a in Influx Transport
CNS ECs express a series of solute transporters that transport specific nutrients such as glucose (GLUT1/Slc2a1), lactate (MCT1/Slc16a1), amino acids (Slc7a1, Slc7a5) into the brain. Mouse null knockout models have been generated for several different transporters, however, these often have phenotypes throughout the organism, as diverse cell types often require transport of these nutrients. For instance, Slc7a1 knockout mice die in the neonatal period with severe anemia, whereas Slc7a5 knockout mice display embryonic lethality. GLUT1 has been largely studied for its role in the delivery of glucose to the CNS. Glucose is the primary source of energy for the brain, and human GLUT1 deficiency results in an epileptic syndrome. A Glut1 knockout allele has been generated with a targeted disruption of the promoter and exon1 of the gene.
We Help You to Clone Genes "Knockout" Mice
Gene-targeted knockout mice are a powerful experimental system for studying development, behavior, and physiology. It also a useful model for systematic analysis of CNS transport from molecular biology.
Why Us
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We have many years' experience and our laboratory is equipped with the very latest instrumentation, so we can offer you the highest quality, accuracy, and precision, and a fast turnaround to meet deadlines.
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We offer comprehensive services across every stage of product development. We're known for our deeply rooted psychotropic drug development expertise, with a global team dedicated to bringing innovations to the market.
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Our goal is to provide the optimal test mix and specialized services to successfully meet the requirements of your trial in the most effective and cost-efficient manner possible.
Our team is what makes Creative Biolabs unique. We have 90% retention of our employees, a management team with an average of 10+ years of industry experience. We strongly believe that the technological advances we make should be accessible to everyone. The process of knowledge exchange is invaluable. Use our expertise to boost your research. If you have any questions, please contact us.
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